Moxi V

Mini Automated Cell Analyzer The Moxi V provides a combination of volumetric cell sizing (Particle Sizer) with simultaneous fluorescence (Cell Analyzer) to provide the most accurate cell counts, size, and viability in the industry.

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Description

Description

Gold Standard Cell Counts, Cell Size, and Viability

The Moxi V provides a combination of volumetric cell sizing (Particle Sizer) with simultaneous fluorescence (Cell Analyzer) to provide the most accurate cell counts, size, and viability in the industry. Specifically, the Moxi V employs the Coulter Principle to precisely measure the volumetric particle size of each particle for exact size measurements down to 3um in diameter (14fL volume), easily distinguishing between cells and debris. The system is also equipped with a 532nm laser and a 561nm/LP detection channel for robust cell viability analysis. Propidium Iodide (PI)-stained dead cells measure 50-100 times brighter fluorescence on the system than do live cells, removing the ambiguity associated with traditional Trypan Blue viability assessments. For each test, these size and viability measurements are applied, individually, to up to 23,000 cells in a matter of a few seconds. This ensures the highest level of precision and statistical robustness. As the fluidic volume is precisely metered as well, the particle counts are presented as an exact cell concentration.

The Moxi V employs a patented, single-use, microfluidic flow cell. The flow cells eliminate the hassle of traditional Cell Analyzers and Coulter Counters, eliminating the need for cleaning, maintenance, clearing of clogs, cross-contamination, and occasionally replacement of bottles and tubes. The Moxi V uses very little sample volume, 60ul, allowing you to conserve your precious, potentially expensive, sample (e.g. stem cells). Cell concentrations as low as 10 cells/ul are possible, typically requiring just 5ul of cell sample diluted in 55ul of PBS.

The Moxi V™ system comes standard with an ultra-intuitive, plug-and-play interface with free OS updates for as long as you own the instrument. No

 

prior Cell Analysis experience is required – you simply just plug and play.

Some Key Features of the Moxi V include:

  • True Cell Viability Counts – 50-100x more sensitive than vision counters.
  • Precision Sizing – Uses the Coulter Principle to get precise cell volumes with CVs less than 3% and no need for triplicates.
  • Highly Accurate – Accurate counts for smaller cells down to 3um (i.e. nuclei, RBCs). Uniquely accurate at low cell concentrations down to 10 cells per ul.
  • Rapid Assays – Offers a less than 15-second test that counts up to 23,000 cells compared to 200-300 counts on most vision counters.

 

Specifications

Specifications

 

ID MXV102
Included Accessories USB power cord, US-style USB power adapter, and Type S+ cassette pack
AC Power Type 110 VAC
Applications Gold Standard Cell Count, Cell Size, and Viability
Average Cell Diameter Range 4 – 25 microns Type M|3 – 20 microns Type S
Battery Type Rechargeable 3.7 V, 7500 mAh lithium ion
Cassette Types Type S+
Cell Particle Concentration Range 10,000 – 5,000 cells/mL Type S+
Cell Types Tested HEK-293|HeLa|PC12|CD3+T|CHO-K1|Cos-7|HepG2|Hybridoma|Jurkat E6-1|K562|MCF7|Mesenchymal SC |Monocyte | Mouse ESC| NIH 3T3| PBMC (cultured)|Red Blood Cells (RBC)|L5178y| C. albicans (Yeast)| S. cerevisiae Vin 13 (Yeast)|S. cerevisiae X5 (Yeast)|Wine Yeast (natural fermentaion)| S.cerevisiae (Baker’s Yeast | Safale US-05 Yeast|
Data Output Formats FCS 3.1, screen shots (.bmp), CSV
Data Storage Capacity 4Gb
Display Resolution 800 x 480 color touchscreen
Excitation Wavelengths 532nm
In British Units 8 lbs
Intended Use Statement For Research Use Only. Product is not for use in diagnostic procedures
Laser Color Green
Measurable Dynamic Range 3 – 27 microns Type Type S+; 4 – 35 microns Type MF-M
Measurement Time 10 seconds Type S+
MPI Cell Health Ratio Yes (Size histogram only)
Number of Detection Channels Flow parameters 1 color, 1 size, 1 forward extinction
Number of PMTs 1
Optical Detection Range 561nm/LP (e.g. PI)
Particle Size Detection Method Impedimetric (Coulter Principle)
Pre-Programmed Tests Gold Standard Cell Count, Cell Size, and Viability
Sample Type Cell Preparations
Sample Volume 60 µL
Supported Connectivity USB on-the-go
Useable Cell Volume 14 – 10,306 fL Type S+; 14 – 22,449 fL Type MF-M
Weight 3.6 kg
Cassettes

Cassettes

Cell Analysis Cassettes

At the core of all of Orflo’s instrument platforms are precision micro-optic microelectronic, microfluidic disposable flow cells. The cassettes eliminate the need for cleaning, maintenance, and optical system alignment and are ideal for safely containing your sample.

Type S+ Cassettes

Type S+ cassettes are designed for cells and particles ranging from 3 “micro-m to 26 um in diameter. Run two Cell Analysis tests with each cassette. 4 parameter outputs per test; 2 colors, cell count, and cell volume.

  • Type S+ Pack (3 packs of 25 Cassettes)
  • Type S+ Box (250 Cassettes)

Type M+ Cassettes

Type M+ cassettes are designed for cells and particles ranging from 4 µm to 34 µm in diameter. Run two Cell Analysis tests with each cassette. 3 parameter outputs per test; 1 color, cell count, and cell volume.

  • Type M+ Pack (3 packs of 25 Cassettes)
  • Type M+ Box (250 Cassettes)
Assay Kits & Reagents

Assay Kits & Reagents

Assay Kits

1. Moxi Cyte Viability (PI)

Propidium Iodide (PI) is a fluorescent dye that is impermeable to healthy cells. When cells die, their membranes become permeable. PI enters the cell, binds to the DNA, and increases fluorescence 50x +. This strong signal for measuring cell viability has made PI the gold-standard approach used in flow cell viability assessment.

2. Orflo Moxi Cyte Nucleated Counts (AO) / Viability (PI) Reagent

The MXA069 reagent is designed for the identification and enumeration of all nucleated cells (Acridine Orange, membrane permeable) in a sample, while simultaneously assessing viability (Propidium Iodide, cell impermeant).

3. Orflo Early Stage Apoptosis Kit (FITC Annexin V / PI)

The MXA701 Early Stage Apoptosis kit is designed to identify early-stage apoptosis in cells by monitoring the phosphatidylserine exposure on the exterior plasma membrane, a hallmark of apoptosis. PI is added to further discriminate the necrotic cells.

 

Reagents

1. System Check Beads, Orange/Red Channel

Utilize the System Check Beads, Orange/Red Channel to periodically verify the proper function of the Moxi GO II PMT2 (561nm/LP, orange/red) channel and the Moxi V. You must flow the System Check Bead protocol to ensure accurate results.

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