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Moxi GO II

Mini Automated Cell Counter Kit Combines the Coulter Principle (for highly-accurate cell counts and exact, volumetric cell sizing) with a 488nm laser and two PMT fluorescence detection channels (for cell health assays, robust CAR-T monitoring, cellular response profiling, and immune profiling).

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Category: Automated Cell Counter

Description

Next Generation Coulter-Principle Cell Analyzer.

Combines the Coulter Principle (for highly-accurate cell counts and exact, volumetric cell sizing) with a 488nm laser and two PMT fluorescence detection channels (for cell health assays, robust CAR-T monitoring, cellular response profiling, and immune profiling).

A small footprint enables portability and convenience.
Fast (<10 sec) test times and no warm-up periods ensure rapid results.
Intuitive, touchscreen design for ease of use.
No cleaning or maintenance is required.

The Moxi GO II’s new Auto-Gating feature will analyze results in an accurate, repeatable way to provide the most consistent results — presented in a simplified new Data Summary page. The new Batch Mode feature allows you to run multiple tests of the same sample type. It will auto-find live and dead cell populations, eliminating user-to-user variability.

Specifications

ID	MXG102
Included Accessories	USB power cord, US-style USB power adapter, and Type S+ cassette pack
AC Power Type	110 VAC
Applications	Mulitplexed Bead ELISA’s\|In Cell Westerrns\|In Cell Protein Quant\|GFP\| Gold Standard Cell Count and Viability\|Mito Potential\|ROS\|Phagocytosis
Battery Type	Rechargeable 3.7 V, 7500 mAh lithium ion
Cassette Types	Type S+
Cell Particle Concentration Range	5,000 – 1,000,000 cells/mL Type S+
Cell Types Tested	HEK-293\|HeLa\|PC12\|CD3+T\|CHO-K1\|Cos-7\|HepG2\|Hybridoma\|Jurkat E6-1\|K562\|MCF7\|Mesenchymal SC\|Monocyte\|Mouse ESC\| NIH 3T3\| PBMC (cultured)\|Red Blood Cells (RBC)\|L5178y\| C. albicans (Yeast)\| S. cerevisiae Vin 13 (Yeast)\|S. cerevisiae X5 (Yeast)\|Wine Yeast (natural fermentaion)\|S.cerevisiae (Baker’s Yeast\|Safale US-05 Yeast\|
Data Output Formats	FCS 3.1, screen shots (.bmp), CSV
Data Storage Capacity	4Gb
Display Resolution	800 x 480 color touchscreen
Excitation Wavelengths	488nm
In British Units	10 lbs
Intended Use Statement	For Research Use Only. Product is not for use in diagnostic procedures
Laser Color	Blue
Measurable Dynamic Range	3 – 27 microns Type Type S+; 4 – 35 microns Type MF-M
Measurement Time	10 seconds Type S+
MPI Cell Health Ratio	Yes (Size histogram only)
Number of Detection Channels Flow parameters	2 color, 1 size, 1 forward extinction
Number of PMTs	2
Optical Detection Range	525/45nm (e.g. FITC, GFP) and 561nm/LP (e.g. PE, RFP)
Particle Size Detection Method	Impedimetric (Coulter Principle)
Platform	Open platform: 561nm/LP (PI, PE, DS Red, Sytox Orange, 7 AAD, Nile Red, Rhodamine Red, Sun Coast Yellow, PE/Cy5), 525/45nm (FITC, GFP, Alexa Fluor 488nm, Calcein)
Pre-Programmed Tests	Mulitplexed Bead ELISA’s\|In Cell Westerrns\|In Cell Protein Quant\|RFP\|Gold Standard Cell Count and Viability\|Mito Potential\|ROS\|Phagocytosis
Resolution Histogram Bins	1000
Sample Type	Beads\|Cell Preparations
Sample Volume	60 µL
Supported Connectivity	USB on-the-go
Useable Cell Volume	14 – 10,306 fL Type S+; 14 – 22,449 fL Type MF-M
Weight	4.53 kg

Cassettes

Cell Analysis Cassettes

At the core of all of Orflo’s instrument platforms are precision micro-optic microelectronic, microfluidic disposable flow cells. The cassettes eliminate the need for cleaning, maintenance, and optical system alignment and are ideal for safely containing your sample.

Type S+ Cassettes

Type S+ cassettes are designed for cells and particles ranging from 3 “micro-m to 26 um in diameter. Run two Cell Analysis tests with each cassette. 4 parameter outputs per test; 2 colors, cell count, and cell volume.

Type S+ Pack (3 packs of 25 Cassettes)
Type S+ Box (250 Cassettes)

Type M+ Cassettes

Type M+ cassettes are designed for cells and particles ranging from 4 µm to 34 µm in diameter. Run two Cell Analysis tests with each cassette. 3 parameter outputs per test; 1 color, cell count, and cell volume.

Type M+ Pack (3 packs of 25 Cassettes)
Type M+ Box (250 Cassettes)

Assay Kits & Reagents

Assay Kits

1. Moxi Cyte Viability (PI)

Propidium Iodide (PI) is a fluorescent dye that is impermeable to healthy cells. When cells die, their membranes become permeable. PI enters the cell, binds to the DNA, and increases fluorescence 50x +. This strong signal for measuring cell viability has made PI the gold-standard approach used in flow cell viability assessment.

2. Orflo Moxi Cyte Nucleated Counts (AO) / Viability (PI) Reagent

The MXA069 reagent is designed for the identification and enumeration of all nucleated cells (Acridine Orange, membrane permeable) in a sample, while simultaneously assessing viability (Propidium Iodide, cell impermeant).

3. Orflo Early Stage Apoptosis Kit (FITC Annexin V / PI)

The MXA701 Early Stage Apoptosis kit is designed to identify early-stage apoptosis in cells by monitoring the phosphatidylserine exposure on the exterior plasma membrane, a hallmark of apoptosis. PI is added to further discriminate the necrotic cells.

Reagents

1. System Check Beads, Orange/Red Channel

Utilize the System Check Beads, Orange/Red Channel to periodically verify the proper function of the Moxi GO II PMT2 (561nm/LP, orange/red) channel and the Moxi V. You must flow the System Check Bead protocol to ensure accurate results.

2. System Check Beads, Green Channel

Utilize the System Check Beads, Green Channel to periodically verify the proper function of the PMT1 (525/45nm, green) channel on the Moxi GO II. You must flow the Moxi GO II System Check Bead protocol to ensure accurate results

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Moxi GO II

Moxi GO II

Description

Next Generation Coulter-Principle Cell Analyzer.

Specifications

Cassettes

Cell Analysis Cassettes

Type S+ Cassettes

Type M+ Cassettes

Assay Kits & Reagents

Assay Kits

1. Moxi Cyte Viability (PI)

2. Orflo Moxi Cyte Nucleated Counts (AO) / Viability (PI) Reagent

3. Orflo Early Stage Apoptosis Kit (FITC Annexin V / PI)

Reagents

1. System Check Beads, Orange/Red Channel

2. System Check Beads, Green Channel

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